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N-Nitroso-N-ethylurea is a precursor of Diazoethane with alkylating, carcinogenic, and mutagenic properties.A precursor of Diazoethane
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Image Search Results
Journal: Journal of Bone and Mineral Research
Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles
doi: 10.1359/jbmr.091023
Figure Lengend Snippet: Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and PHOSPHO1-deficient (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”
Article Snippet: The
Techniques: Activity Assay
Journal: Journal of Bone and Mineral Research
Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles
doi: 10.1359/jbmr.091023
Figure Lengend Snippet: Amounts of hydrolyzed ATP and produced ADP and AMP by MVs: WT (•), TNAP-deficient (○), NPP1-deficient (□), and PHOSPHO1-deficient (Δ), monitored by HPLC analysis as described in “Materials and Methods.”
Article Snippet: The
Techniques: Produced
Journal: Journal of Bone and Mineral Research
Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles
doi: 10.1359/jbmr.091023
Figure Lengend Snippet: Amounts of hydrolyzed ADP and produced AMP for MVs: WT (•), TNAP-deficient (○), NPP1-deficient (□), and PHOSPHO1-deficient (Δ), monitored by HPLC analysis as described in “Materials and Methods.”
Article Snippet: The
Techniques: Produced
Journal: Journal of Bone and Mineral Research
Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles
doi: 10.1359/jbmr.091023
Figure Lengend Snippet: Schematic representation of the most favored enzymatic reactions in the MV compartment at physiologic pH. We surmise that HA seed crystals are formed in the sheltered interior of MVs favored by the P i -generating ability of PHOSPHO1, as well as by the function of Pit1/2 phosphate transporters. While NPP1 clearly has a role in PP i -generation at the level of the chondrocyte and osteoblast membranes, at the level of MVs NPP1 does not use ATP efficiently. Instead, it is TNAP that has a major role both as a PP i ase and an ATPase/ADPase and thus participates in the calcification process both by restricting the concentration of extracellular PP i and by also contributing to the P i pool available for calcification. Thick lines indicate the most favored reactions, whereas dotted lines indicate alternative enzymatic pathways, such as the ability of NPP1 to act as a phosphatase.
Article Snippet: The
Techniques: Concentration Assay